User manual LEICA MM MITOTIC INDEX BROCHURE

[. . . ] Leica MM Mitotic Index Analysis Software Drop-in for Leica MM AF · Quantitation of mitotic and interphase cells · Multi-parameter analysis of two probes · Adaptive Background CorrectionTM for improved segmentation · Cell-by-Cell measurements and data logging Cell-based assays for cell cycle status have become critical for oncology research. [. . . ] The Leica MM Mitotic Index module for the Leica MM AF software is designed for the quantitative discrimination of mitotic and interphase cells. powered by MetaMorph® Cells are labeled with a DNA stain and a fluorescent mitosis-specific marker, such as immunofluorescence staining for Histone 3 S10 phosphorylation. Two different wavelengths are acquired and the images are analyzed with the Leica MM Mitotic module. The module utilizes Adaptive Background Correction adapting the cell detection algorithm to the local intensity ranges between and within cells to provide the most robust segmentation available. This technique enables probe detection even with highly variable background fluorescence within a single image. The simple interface minimizes setup efforts and at the same time enables users to customize the settings and measurements to obtain the best possible results specific to the type of cells used in the experiments. Easy Configuration for analysis 1. Optionally choose the reporting parameters Customization through journaling Journals are sophisticated and powerful macros that record and perform a series of tasks without the need for a programming language. [. . . ] Top: control, bottom: 50 ng/ml Nocodazole. Powerful data export capabilities All measurements can be directly exported to a text file or Microsoft® Excel® for further analysis. "MetaMorph® is a Registered Trademark of MDS Analytical Technologies" Image analysis The Leica MM Mitotic Index module identifies mitotic cells (green) and interphase cells (red). Export data for further analysis www. leica-microsystems. com [. . . ]