User manual LEICA EM RTS BROCHURE

[. . . ] Leica EM PACT2 High Pressure Freezer Leica EM RTS Rapid Transfer System High Pressure Freezing for Everybody The Leica EM PACT2 high pressure freezer serves the needs of molecular and cell biologists and all researchers who want an "in vivo" impression of their cellular structures and functions in question ­ without the artefacts of chemical fixation but with the high resolution information of EM immunocytochemistry, frozen hydrated sections and freeze fracturing. The Rapid Transfer System EM RTS allows correlative LM/EM experiments, taking a live specimen from a light microscope (e. g. Safety and reproducibility for the specimen are increased while operator mistakes are reduced. Structural details of the C. Müller-Reichert (MPI-CBG, Dresden, Germany) and Kent McDonald (University of California, Berkeley, USA) 2 Leica Design by W. [. . . ] After cryofixation the carriers are collected in the LN2 bath of the EM PACT2 before freeze substitution in the Leica EM AFS2. 6 The Tube Holder for every fluid you can think of . . . Directly suck the suspension into copper tubes (inner ø 350 µm) already mounted in their holder. Alternatively, take up sample into cellulose microcapillary. With the loading device the specimen can be inserted into the EM PACT. After cryofixation the samples are stored under LN2 in the transfer box. Sample Punch: punching out of the central part of the specimen tube ready for Frozen Hydrated Sectioning or . . . . . . peeling away of the top of the copper tube for Freeze Substitution. Storage of the copper tubes is carried out under LN2. The specimen tube holders are recycled by reloading them with copper tubes. Leica Design by W. Hölbl 7 Leica EM RTS Application Solutions Taking microbiopsies for EM is now faster than ever before!Prepare the Microbiopsy Transfer Station for RTS under the Optical Workstation. . . and transfer the tissue into the biopsy carrier. The specimen and carrier are held in the rapid loader . . . . . . while the primed loading device waits for freezing in the RTS. By gently pushing the rapid loader into the RTS the carrier is tightened securely and then frozen automatically in less than 2. 5 seconds. After cryofixation the carriers are collected in the LN2 bath of the EM PACT2 before freeze substitution in the Leica EM AFS2 or cryosectioning in the EM FC6. 8 Frozen Hydrated Sectioning made easy. Freeze Substitution) can be conveniently performed in the cryopreparation box supplied with the Leica EM PACT2. The Freeze Fracture Holder for everything that can be fractured . . . A copper ring is put on top of the loaded carrier. The carrier and copper ring are sandwiched securely in the pod with the supplied torque wrench. With the loading device the specimen can be inserted into the instrument. After cryofixation the carriers are collected in the LN2 bath of the EM PACT2 before Freeze Fracturing. Accessories are also available for loading EM PACT2 without EM RTS. 9 Mouse embryonic fibroblast grown on sapphire disc. Soazig Lelay, Jana Maentler and Paul Verkade, MPI-CBG, Dresden, Germany 10 Yeast frozen in the membrane carrier. Courtesy of Mark van Breugel, Jana Maentler and Paul Verkade, MPI-CBG, Dresden, Germany. Insulin granules producing INS-1 cells. Courtesy of Joke Ouwendijk, Jana Maentler, Melanie Jäger, Michele Solimena and Paul Verkade, TUD and MPI-CBG, Dresden, Germany Rat, Langerhans cells. [. . . ] +43 1 48899-235 Fax +43 1 48899-350 Leica EM PACT2_EM RTS - E - 6/05 Order No 206002, Printed on chlorine-free bleached paper. Printed in Austria. Our expertise in microscopy is the basis for all our solutions for visualization, measurement and analysis of microstructures in life sciences and industry. With confocal laser technology and image analysis systems, we provide threedimensional viewing facilities and offer new solutions for cytogenetics, pathology and materials sciences. [. . . ]